In the precise world of protein research, every detail matters. Protein markers, as key tools in biological experiments, are lighting the way forward for researchers with their unique concept of "exquisite positioning".

Definition and function of protein markers

Protein markers are protein standards with pre-set molecular weight and concentration, which are composed of proteins of various molecular weights. During protein electrophoresis, they are electrophoretically separated simultaneously with the protein sample to be tested. Since proteins of different molecular weights have different migration rates in electrophoresis, protein markers can form a series of bands of known molecular weight in the gel. These bands are like a precise ruler, providing key reference standards for researchers to help quickly uate the separation effect and quantitative analysis of proteins.

Features of prestained protein markers

Depending on different experimental requirements, protein markers can be divided into many types. Prestained protein markers are the best among them. They are suitable for protein electrophoresis experiments of staining and decolorization, and have good stability and repeatability. Its pre-stained characteristics allow the migration of bands to be directly observed during electrophoresis, making it convenient to adjust experimental conditions in a timely manner. Moreover, pre-stained protein markers have undergone rigorous purification treatment and are of high purity, which will not interfere with experimental results.

Key points for quality control of protein markers

In order to ensure the accuracy and reliability of protein markers in experiments, their quality needs to be strictly controlled. Protein purity is the primary consideration, and protein purity can be accurately detected by gel electrophoresis, mass spectrometry and other methods. The accuracy of molecular weight is also crucial and needs to be compared and verified with proteins of known molecular weight. In addition, the stability of concentration also needs to be tested regularly to ensure the comparability and repeatability of each experiment.

Application of protein markers in protein electrophoresis

Protein markers are widely used in protein electrophoresis. In SDS-PAGE electrophoresis, it can be electrophoretically separated simultaneously with protein samples to help researchers quickly determine the molecular weight and separation of the target protein. In Western Blot experiments, protein markers can be used as molecular weight standards to help determine the location and size of the target protein. At the same time, it can also be used to uate the transfer efficiency to ensure that the target protein is successfully transferred to the membrane.